Support

No. In fact, even Joomla's own .htaccess won't work with that. Please read the Apache documentation about AllowOverride to understand why.

At the very least you will need to add FileInfo to enable URL redirection used to provide Joomla's SEF URLs and the basic protection (Frontend and Backend protection rules). If you want to use the custom index option in the .htaccess Maker you need to add Indexes. If you want to use blocking of user agents you need to add Limit and possibly AuthConfig, depending on your server version. If you want to apply optimization (caching, compression) and advanced security rules (anything which adds or manipulates HTTP protocol headers) or anything else I have not listed here you need All or you need to go through the generated directives and put everything that's blocked in a custom AllowOverrideList directive in your Apache configuration's Directory statement.

Of course you can sidestep this issue by not using .htaccess. This is better for performance and security but a pain in the posterior when you need to make small changes. Use the .htaccess Maker normally but do NOT generate an .htaccess. Instead, preview it. All of these directives can be placed inside the virtual host (vhost) configuration file of your Apache installation. If I recall correctly all of them can go into the Directory directive inside your vhost configuration. Since they are inside the actual Apache configuration they only need to be read and parsed once, when you (re)start Apache or reload its configuration. This makes things substantially faster for processing requests. The downside is that updating these directives requires editing the Apache configuration and reloading or restarting Apache when you're done.

If you are using the host configuration instead of .htaccess you can set AllowOverride AuthConfig

Remember, AllowOverride is only controlling what .htaccess can do. Inside the vhost confit you can do everything, the permission is implicit there.